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Regeneration of tea [Camellia sinensis (L.) Kuntze] cv. TRI 5001 plants through somatic embryogenesis in a liquid dynamic medium

Authors:

K. K. Ranaweera ,

University of Peradeniya, LK
About K. K.

Postgraduate Institute of Agriculture

 

Plant Breeding Division, Tea Research Institute of Sri Lanka, Talawakelle

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M. A. B. Ranatunga,

Tea Research Institute of Sri Lanka, Talawakelle, LK
About M. A. B.
Plant Breeding Division
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J. P. Eeswara,

University of Peradeniya, LK
About J. P.
Department of Crop Science, Faculty of Agriculture
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S. Thilakarathne,

University of Ruhuna, Kamburupitiya, LK
About S.
Faculty of Technology
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H. Kadono

Saitama University, JP
About H.
Optical Sensing Laboratory, Faculty of Science and Technology
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Abstract

Somatic embryogenesis is an efficient micro-propagation technique, which can lead to mass multiplication of tea with a tap root. Hence, the development of a viable somatic embryogenesis and multiplication protocol through liquid culture conditions is needed to increase the efficiency of the protocol. Cotyledon-derived somatic embryos, leaf and stem callus of TRI 5001 cultivar, were tested with different liquid MS media. A significantly higher cotyledon-derived somatic embryo multiplication rate was observed in MS +3 mg/L Thidiazuron (TDZ) medium. Callus proliferation and compact and embryonic callus formation were comparatively higher in MS + 1.1 mg/L TDZ, 1.86 mg/L NAA medium, and subsequent somatic embryo formation was observed in MS + 0.1 mg/L NAA,1 mg/L BAP and 0.2 mg/L GA3 medium on callus produced on leaf explants. Higher nodal callus proliferation and compact callus formation were recorded in MS+0.11 mg/L TDZ 0.1 mg/L IBA and higher embryonic callus was observed in MS+0.1 mg/L TDZ, 1.86 mg/L NAA media. Plant regeneration was resulted in MS+3 mg/LBAP 0.1 mg/L NAA medium. Identified treatment combinations can be used as a foundation to establish somatic embryogenesis protocol through different explants of tea.
How to Cite: Ranaweera, K.K., Ranatunga, M.A.B., Eeswara, J.P., Thilakarathne, S. and Kadono, H., 2022. Regeneration of tea [Camellia sinensis (L.) Kuntze] cv. TRI 5001 plants through somatic embryogenesis in a liquid dynamic medium. Tropical Agricultural Research, 33(4), pp.364–375. DOI: http://doi.org/10.4038/tar.v33i4.8520
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Published on 01 Oct 2022.
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