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Wild Rice Species in Sri Lanka as Genetic Resources for Breeding for Brown Plant Hopper (Nilaparvata lugens (Stål)) Resistance in Rice

Authors:

A. V. C. Abhayagunasekara,

University of Peradeniya, Peradeniya, LK
About A. V. C.

Postgraduate Institute of Agriculture,

 

Fruit Crops Research and Development Station, Gannoruwa, Peradeniya, Peradeniya

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G. D. S. N. Chandrasena,

Rice Research and Development Institute, Batalagoda, LK
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D. M. O. K. B. Dissanayake,

Rice Research and Development Institute, Batalagoda, LK
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J. M. S. M. Jayasundara,

University of Peradeniya, Peradeniya, LK
About J. M. S. M.
Agricultural Biotechnology Centre, Faculty of Agriculture
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D. K. N. G. Pushpakumara,

University of Peradeniya, Peradeniya, LK
About D. K. N. G.
Department of Crop Science, Faculty of Agriculture
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W. L. G. Samarasinghe,

Ministry of Agriculture, 80/5, “Govijana Mandiraya”, Battaramulla, LK
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P. C. G. Bandaranayake

University of Peradeniya, Peradeniya, LK
About P. C. G.
Agricultural Biotechnology Centre, Faculty of Agriculture
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Abstract

Brown pl anthopper (BPH) Nilaparvata lugens (Stål.) is considered the most destructive rice pest in rice-growing countries  including Sri Lanka. Resistance breeding considered the most e conomical management strategy, starts by identifying new genetic resources for BPH resistance and comparative analysis wi th existing donors. So far, scientists have identified 38 genes/QTLs responsible for BPH resistance. This study explored the BPH resistance in five wild rice species in Sri Lanka, inc luding the endemic species Oryza rhizomatis. One recommended rice variety; Bg 352, fourteen O. rhizomatis accessions, and one accession from Oryza nivara, Oryza rufipogon,  Oryza granulata and Oryza eichingeri screened by the honeydew test. The experiment was arranged in a complete randomize d design with fifteen replicates with Bg 380 and Ptb 33 as susc eptible and resistant checks respectively. The lower amount of honeydew excretion recorded in all tested O. rhizomatis  accessions, O. nivara, O. granulata, and O. eichingeri accessions  suggested BPH resistance. Both Ptb 33 and O. rufipogon showed a similar level of resistance with low honeydew  excretion. The varieties Bg 352 and Bg 380 showed a high amount of honeydew excretions confirming higher susceptibility to the BPH. Therefore, all fourteen accessions of O. rhizomatis and the selected accessions of O. nivara and O. eichingeri are potential donors for rice breeding programs. Further, th e coding sequences (CDS) of known BPH genes of O. sativa were used as queries to search similar genes in wild rice genomes. The CDS coverage and phylogenetic analysis suggest, of known BPH resistance genes, at least BPH6, BPH9, BPH14-1, and BPH18 are present in O. rhizomatis, O. eichingeri, O. nivara, and O. rufipogon genomes. This information will assist in marker-as sisted breeding attempts.
How to Cite: Abhayagunasekara, A.V.C., Chandrasena, G.D.S.N., Dissanayake, D.M.O.K.B., Jayasundara, J.M.S.M., Pushpakumara, D.K.N.G., Samarasinghe, W.L.G. and Bandaranayake, P.C.G., 2022. Wild Rice Species in Sri Lanka as Genetic Resources for Breeding for Brown Plant Hopper (Nilaparvata lugens (Stål)) Resistance in Rice. Tropical Agricultural Research, 33(2), pp.102–112. DOI: http://doi.org/10.4038/tar.v33i2.8550
Published on 30 Mar 2022.
Peer Reviewed

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