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Field Performance of Mixtures of Pseudomonas and Bacillus spp. in Managing Papaya Ringspot Virus Disease and their Effect on Plant Defense Enzyme Activity

Authors:

C. Ranasinghe ,

Postgraduate Institute of Agriculture, University of Peradeniya, LK
About C.
Board of Study-Plant Protection
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D.M. De Costa

Board of Study-Plant Protection, Postgraduate Institute of Agriculture, University of Peradeniya, LK
About D.M.
Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya
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Abstract

The present study was conducted to determine the field efficacy of Pseudomonas and Bacillus spp., isolated from the rhizosphere of healthy papaya to manage papaya ringspot virus disease (PRSVD), when applied as isolate-mixtures along with their effect on plant defense enzyme activity.  Mixtures of bacterial isolates were applied by two methods, namely seed soak (SS) and soil drench (SD) to field-grown and naturally-infected papaya plants (var. Red Lady). Disease severity and fruit yield were quantified over a period of six months and defense-related enzyme activity in leaves was quantified with spectrophotometry.  At the sixth month after transplanting, SS-treated plants with the mixture of Bacillus isolates and the combined mixture of Pseudomonas and Bacillus isolates showed a significantly lower % disease index on foliage than the plants under control and the Pseudomonas mixture treatments. A significantly lower % disease index was shown on the fruits treated with all the bacterial treatments applied by both methods, in comparison to the untreated control. Mixture of Pseudomonas isolates and the combined treatment with Pseudomonas and Bacillus isolates, when applied as the SS method, gave significantly higher fruit yield than that of the control treatment. Activities of peroxidase and phenylalanine ammonia lyase were significantly higher in plants treated with the mixture of Pseudomonas isolates by SS method while β-1,3-glucanase activity was significantly higher when applied the same treatment by SD method. Findings revealed the ability to reduce severity of PRSVD and induce defense-related enzymes when mixtures of Pseudomonas and Bacillus spp. were applied under field conditions.
How to Cite: Ranasinghe, C. and De Costa, D.M., 2020. Field Performance of Mixtures of Pseudomonas and Bacillus spp. in Managing Papaya Ringspot Virus Disease and their Effect on Plant Defense Enzyme Activity. Tropical Agricultural Research, 31(2), pp.75–85. DOI: http://doi.org/10.4038/tar.v31i2.8369
Published on 01 Apr 2020.
Peer Reviewed

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