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Effect of Phenological Growth Stage on Establishment of In-vitro Cultures of Bael (Aegle marmelos (L.) Corr.)

Authors:

C. K. Pathirana ,

University of Peradeniya, LK
About C. K.
Postgraduate Institute of Agriculture
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A. M. U. R. K. Attanayake,

University of Peradeniya, LK
About A. M. U. R. K.
Department of Crop Science, Faculty of Agriculture
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D. M. U. S. K. Dissanayake,

University of Peradeniya, LK
About D. M. U. S. K.
Department of Crop Science, Faculty of Agriculture
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J. G. K. L. Gamlath,

Fruit Crop Research & Development Institute, Gannoruwa, Peradeniya, LK
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K. W. Ketipearachchi,

Fruit Crop Research & Development Institute, Gannoruwa, Peradeniya, LK
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T. Madhujith,

University of Peradeniya, LK
About T.
Department of Food Science and Technology, Faculty of Agriculture
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P. C. G. Bandaranayake,

University of Peradeniya, LK
About P. C. G.
Agricultural Biotechnology Centre, Faculty of Agriculture
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J. P. Eeswara

University of Peradeniya, LK
About J. P.
Department of Crop Science, Faculty of Agriculture
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Abstract

Bael (Aegle marmelos (L.) Corr.) is a medicinal fruit tree species belongs to the family Rutaceae grown in South Asian countries including India, Sri Lanka and Bangladesh. It is an underutilized fruit species in Sri Lanka, although it has food as well as medicinal value with a good economic potential. Popularizing bael as a profitable cash crop is often hindered by the limited availability of high quality planting material. In Sri Lanka, five elite bael accessions namely Beheth Beli, Paragammana, Mawanella, Rambukkana and Polonnaruwa Supun have been identified and used for mass propagation through budding and grafting. But this effort is often hampered by many limitations faced in large scale production. Micropropagation is an alternative technique to produce clonal plants in large scale. However, the complex phenological behaviour of the bael trees could affect its success. Therefore, the present study was undertaken to identify the correct phenological stage of bael trees to collect explants for the micropropagation. Leaves and twig explants were collected for micropropagation from five elite bael accessions during the period of July, 2016 to June, 2017 on monthly basis to capture the best phenological stage. The Beheth Beli tree and grafted plants of other four accessions were established at the Fruit Crop Research and Development Institute, Department of Agriculture, Sri Lanka. The surface sterilized explants were established on Murashige and Skoog medium supplemented with 1 mg/L of 6-Benzylaminopurine, 3% sucrose, solidified with phytogel. A successful organogenesis was only observed in explants collected in the months of April, May and June (39 to 68 %) where there was no significant difference in success was observed between the leaf and twig explants (P>0.05). However, grafted bael accessions exhibited a significantly different mean success percentage in organogenesis where Paragammana and Rambukkana accessions showed a high success for leaf explants and Mawanella and Rambukkana accessions showed a high success for twig explants. These results could be readily employed to multiply the elite bael accessions in Sri Lanka.
How to Cite: Pathirana, C.K., Attanayake, A.M.U.R.K., Dissanayake, D.M.U.S.K., Gamlath, J.G.K.L., Ketipearachchi, K.W., Madhujith, T., Bandaranayake, P.C.G. and Eeswara, J.P., 2018. Effect of Phenological Growth Stage on Establishment of In-vitro Cultures of Bael (Aegle marmelos (L.) Corr.). Tropical Agricultural Research, 29(3), pp.268–275. DOI: http://doi.org/10.4038/tar.v29i3.8266
Published on 28 Jun 2018.
Peer Reviewed

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