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Research Articles

Molecular diversity analysis of conserved Capsicum chinense Jacq. germplasm in Sri Lanka

Authors:

N. H. L. D. L. D. Nanayakkara,

University of Peradeniya, LK
About N. H. L. D. L. D.
Postgraduate Institute of Agriculture
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S. K. Wasala ,

Plant Genetic Resources Center, Gannoruwa, LK
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N. M. Ubeysekara,

Plant Genetic Resources Center, Gannoruwa, LK
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K. G. C. N. Jayarathne,

Plant Genetic Resources Center, Gannoruwa, LK
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I. P. Wickremasinghe

University of Peradeniya, LK
About I. P.
Department of Agricultural Biology, Faculty of Agriculture
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Abstract

Among the Capsicum species, Capsicum chinense has the highest pungency, the highest antioxidant activity and a wide genetic diversity. This study was conducted to evaluate the genetic diversity of C. chinense (Nai miris) germplasm conserved at the Plant Genetic Resources Centre, Gannoruwa, Sri Lanka. Twenty five C .chinense germplasm, two C. annuum, two C. frutescens and one C. baccatum were analysed using 27 Simple Sequence Repeat markers. Total number of amplified alleles was 108 which varied from 1 to 6 per locus. The mean polymorphism information content value was 0.46. Dendogram based on Nei’s genetic distances showed three main clusters where 88% of C. chinense germplasm was grouped into one cluster and C. annuum and C. frutescens were separated into two clusters. A high genetic diversity was observed within C. chinense cluster. The genetic diversity identified in this study will be useful for correct identification, conservation and breeding activities of Capsicum species.

How to Cite: Nanayakkara, N.H.L.D.L.D., Wasala, S.K., Ubeysekara, N.M., Jayarathne, K.G.C.N. and Wickremasinghe, I.P., 2018. Molecular diversity analysis of conserved Capsicum chinense Jacq. germplasm in Sri Lanka. Tropical Agricultural Research, 29(2), pp.218–224. DOI: http://doi.org/10.4038/tar.v29i2.8291
Published on 29 May 2018.
Peer Reviewed

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