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Effect of Gibberellic Acid (GA3) and Sugar on In Vitro Cormlet Formation, Multiplication and Ex Vitro Sprouting of Gladiolus hybrida Variety Princess Lee

Authors:

PAIU Dharmasena,

Postgraduate Institute of Agriculture, University of Peradeniya, LK
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DP Karunananda,

Horticultural Research and Development Institute, Gannoruwa, Peradeniya, LK
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JP Eeswara

Department of Crop Science, Faculty of Agriculture, University of Peradeniya,, LK
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Abstract

Calli were initiated from corms of Gladiolus hybrida variety Princess Lee by culturing on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l napthaline acetic acid (NAA) and 20 g/l sucrose. In vitro shoots were regenerated from the calli by transferring into MS medium containing 30 g/l sucrose without growth regulators. Regenerated shoots produced cormlets at the base of the shoot on full or half strength MS medium supplemented with 40-60 g/l sucrose. Highest number of large corms (diameter>1.5 cm) was obtained in the half strength MS medium containing 60 g/l sucrose. Multiplication of in vitro-grown cormlets was achieved by transferring them on to half strength MS medium containing 60 g/l sucrose with 5 or 10 mg/l gibberellic acid (GA3). Multiplied corms were successfully acclimatized on a medium containing soil: sand: brick pieces at 1:1:1 ratio. Acclimatized cormlets were established on two different potting mixtures i.e. sand: leaf mould: brick pieces (1:1:1) or sand: compost: brick pieces (1:1:1) after pre-treating with or without 100 mg/l GA3 for 24 hours. Early sprouting (13 days) was observed in cormlets treated with 100 mg/l GA3 compared to cormlets without any GA3 treatment (28 days) and, the highest sprouting percentage (83.3 %) was observed on potting mixture containing sand, leaf mould and brick pieces (1:1:1) under greenhouse conditions.

Tropical Agricultural Research Vol. 23 (1): 1–10 (2011)

DOI: http://dx.doi.org/10.4038/tar.v23i1.4626
How to Cite: Dharmasena, P., Karunananda, D. and Eeswara, J., 2012. Effect of Gibberellic Acid (GA3) and Sugar on In Vitro Cormlet Formation, Multiplication and Ex Vitro Sprouting of Gladiolus hybrida Variety Princess Lee. Tropical Agricultural Research, 23(1), pp.1–10. DOI: http://doi.org/10.4038/tar.v23i1.4626
Published on 06 Sep 2012.
Peer Reviewed

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