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A preliminary investigation on methods of reducing antibiotic resistant bacteria in broiler litter in selected farms in mid country, Sri Lanka

Authors:

E.M. Herath ,

University of Peradeniya, LK
About E.M.
Postgraduate Institute of Agriculture
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W.S. Dandeniya,

University of Peradeniya, LK
About W.S.
Department of Soil Science, Faculty of Agriculture
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A.G.S.I. Samarasinghe,

University of Peradeniya, LK
About A.G.S.I.
Faculty of Veterinary Medicine & Animal Science
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T.P.M.S.D. Bandara,

University of Peradeniya, LK
About T.P.M.S.D.
Faculty of Veterinary Medicine & Animal Science
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R.N. Jinadasa

University of Peradeniya, LK
About R.N.
Faculty of Veterinary Medicine & Animal Science
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Abstract

Antibiotic residues and bacteria resistant to antimicrobial have been found in poultry litter, which is popular low cost manure in agriculture. A study was conducted to investigate a pre-treatment method to reduce antibiotic resistant bacteria in poultry litter. Poultry litter was collected from eleven medium-scale broiler farms in Kandy. Total and antibiotic resistant (tetracycline and/or enrofloxacin) culturable bacteria were isolated using Tryptic Soy Agar supplemented with 0 or 100 ppm of each antibiotic. A fresh and a cured broiler litter sample were collected from one farm and treated with different temperatures (25°C, 40°C, 60°C and 80°C for 24 hours), neem seed extract (10 and 1000 ppm for 4 day) and neem dry leaves (10% w/w for 4 days) in triplicates. Total and antibiotic resistant bacteria in treated litter were assessed. Broiler litter from all farms contained tetracycline resistant bacteria. Heating at 80°C reduced tetracycline resistant bacteria by 96-100% compared to 25°C. Neem seed extract at 10 ppm reduced tetracycline resistant bacteria by 75-96%. Treating broiler litter with high temperature (>60°C) and neem seed extract (10ppm) could minimize spread of antibiotic resistant bacteria to the environment.

Tropical Agricultural Research Vol. 26 (2): 409 – 414 (2015)

DOI: http://doi.org/10.4038/tar.v26i2.8104
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Published on 20 Nov 2015.
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