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Reading: Overexpression of CnANT, Coconut BABYBOOM Homologue Alters Plant Growth and Morphology in Tr...

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Overexpression of CnANT, Coconut BABYBOOM Homologue Alters Plant Growth and Morphology in Transgenic Arabidopsis Plants

Authors:

HDD Bandupriya ,

School of Biological Sciences, University of Reading, GB
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JM Dunwell

School of Biological Sciences, University of Reading, GB
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Abstract

Identification of molecular markers for the recognition of tissues competent  for in vitro regeneration has a considerable potential for increasing the efficiency of coconut  somatic embryogenesis protocol. BABYBOOM (BBM), APETALA2/Ethylene Responsive  Element Binding Protein (AP2/EREBP) family gene has shown a role during embryogenesis.  To gain insight into the functions of CnANT, a BABYBOOM gene homologue of coconut was  cloned under the cauliflower mosaic virus-35s promoter and the plant growth and  developmental changes that occur in transgenic Arabidopsis plants were determined and  compared with the data obtained for other BBM genes originated from other plant species.  Construction of vectors for Arabidopsis transformation was based on pGREEN plasmid.  Transformed plants were screened by spraying herbicide Harvest which contains the active  ingredient gluphosinate ammonium. Five independent transgenic lines were developed. All  lines showed the typical 3:1 segregation ratio suggesting that the bar genes had integrated  into the Arabidopsis as a single segregating locus. In comparison to wild type plants,  overexpression of CnANT resulted in pleiotropic phenotypes including compact rosettes,  backward curly-leaves with serrated margins, larger flowers and smaller siliques. There  were also modifications in plant growth such as delay in flowering. Similar phenotypic  effects observed for CnANT along with BBM and other related genes from eudicots suggest  similar biochemical targets governed by these proteins. Similarity of their expression, thus  suggest that CnANT gene also has a potential to increase in vitro regeneration response.

Tropical Agricultural Research Vol. 23 (3): 249-260 (2012)

DOI: http://dx.doi.org/10.4038/tar.v23i3.4662

 

How to Cite: Bandupriya, H. & Dunwell, J., (2012). Overexpression of CnANT, Coconut BABYBOOM Homologue Alters Plant Growth and Morphology in Transgenic Arabidopsis Plants. Tropical Agricultural Research. 23(3), pp.249–260. DOI: http://doi.org/10.4038/tar.v23i3.4662
Published on 19 Sep 2012.
Peer Reviewed

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