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Effect of Gibberellic Acid (GA3) and Sugar on In Vitro Cormlet Formation, Multiplication and Ex Vitro Sprouting of Gladiolus hybrida Variety Princess Lee

Authors:

PAIU Dharmasena,

Postgraduate Institute of Agriculture, University of Peradeniya, LK
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DP Karunananda,

Horticultural Research and Development Institute, Gannoruwa, Peradeniya, LK
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JP Eeswara

Department of Crop Science, Faculty of Agriculture, University of Peradeniya,, LK
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Abstract

Calli were initiated from corms of Gladiolus hybrida variety Princess Lee by culturing on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l napthaline acetic acid (NAA) and 20 g/l sucrose. In vitro shoots were regenerated from the calli by transferring into MS medium containing 30 g/l sucrose without growth regulators. Regenerated shoots produced cormlets at the base of the shoot on full or half strength MS medium supplemented with 40-60 g/l sucrose. Highest number of large corms (diameter>1.5 cm) was obtained in the half strength MS medium containing 60 g/l sucrose. Multiplication of in vitro-grown cormlets was achieved by transferring them on to half strength MS medium containing 60 g/l sucrose with 5 or 10 mg/l gibberellic acid (GA3). Multiplied corms were successfully acclimatized on a medium containing soil: sand: brick pieces at 1:1:1 ratio. Acclimatized cormlets were established on two different potting mixtures i.e. sand: leaf mould: brick pieces (1:1:1) or sand: compost: brick pieces (1:1:1) after pre-treating with or without 100 mg/l GA3 for 24 hours. Early sprouting (13 days) was observed in cormlets treated with 100 mg/l GA3 compared to cormlets without any GA3 treatment (28 days) and, the highest sprouting percentage (83.3 %) was observed on potting mixture containing sand, leaf mould and brick pieces (1:1:1) under greenhouse conditions.

Tropical Agricultural Research Vol. 23 (1): 1–10 (2011)

DOI: http://dx.doi.org/10.4038/tar.v23i1.4626
How to Cite: Dharmasena, P., Karunananda, D. & Eeswara, J., (2012). Effect of Gibberellic Acid (GA3) and Sugar on In Vitro Cormlet Formation, Multiplication and Ex Vitro Sprouting of Gladiolus hybrida Variety Princess Lee. Tropical Agricultural Research. 23(1), pp.1–10. DOI: http://doi.org/10.4038/tar.v23i1.4626
Published on 06 Sep 2012.
Peer Reviewed

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